Seminar

Mechanistic models of endocytosis based on the structural studies on endocytic proteins

Dr. Atsushi Shimada

The internalization of extracellular molecules through clathrin-mediated endocytosis (CME) is crucial in many physiological responses in eukaryotes, such as receptor internalization, nutrient uptake, and synaptic vesicle recycling. CME starts with the assembly of clathrin into the clathrin lattice on the membrane. During clathrin assembly, clathrin does not directly interact with the membrane and requires a number of cytosolic regulatory proteins for higher-order assembly on the membrane. After clathrin assembly, the clathrin-coated pits invaginate for CME to further proceed. In this talk, I discuss the mechanistic models of clathrin assembly and membrane invagination based on the structural and functional data on some of the related regulatory proteins of CME1,2. References 1. Shimada, A. Yamaguchi, A., Kohda, D. Structural basis for the recognition of two consecutive mutually interacting DPF motifs by the SGIP1 ? homology domain., Sci. Rep., 6, 19565 (2016). 2. Shimada, A. et al., Curved EFC/F-BAR domain dimers are joined end to end into a filament for membrane invagination in endocytosis, Cell, 129, 761-772 (2007).