Technologies

The scientific and technical staff at the center is expert in providing solutions to a large variety complex scientific problems and questions raised from industry partners, encompassing the diverse number of disciplines and technologies widely used in life sciences and biomedical research. We also implement and develop state-of-the-art biotechnological tools and reagents for research through collaborations or service provision.

In line with our spirit for solving challenges and fostering collaborations, we are always open to new those scientific and technical challenges that require advanced solutions. For specific needs of not listed technologies or services, please contact our Technology Transfer Manager or get in touch with Technology Platforms of your interest directly.

Target Identification Analysis

With tools such as BioE3, UbL-ID, BioUbL, Molecular Traps (TUBES, SUBES) and TurboID, coupled to label-free quantitative proteomics, we analyse protein-protein interactions and modifications by ubiquitin (Ub) and ubiquitin-like proteins (UbLs)

BioE3
Identification of bona-fide targets of E3 ligases of the ubiquitin family.
Interactors
Identification of proximal interactors by proteomics.
Molecular Traps
Isolation of endogenous proteins modified by the ubiquitin family (ubiquitin and SUMO).
UbL-ID
Identification of interactors of proteins modified by the ubiquitin family.
BioUbL
General identification of proteins modified by the ubiquitin family.

Detailed info Link

For specific needs, please contact our Technology Transfer Manager or Rosa Barrio or James D Sutherland

Extracellular Vesicles Deep Analysis

Extracellular vesicles including exosomes are secreted by cells of all kingdoms as an intercellular communication mechanism. These vesicles have found great biomedical interest in diagnostics as a source of non-invasive biomarkers, and also in therapy by their intrinsic therapeutics properties and their capabilities to be engineered as specific drug-delivery vehicles. We offer an integral solution to analyze vesicle derived from a plethora of different matrices (Serum, Urine, Tissues, Cell Culture, etc.)

Functional Analysis
Uptake, Bioenzymatic activity and Biodistribution in vivo

Molecular Characterization
Single Vescicle Distribution, Molecular Characterization (Inmuno-assays, omics analysis)

For specific needs, please contact our Technology Transfer Manager or Juan Manuel Falcon

Multi-omic integration

Our platform for multi-omic integration leverages cutting-edge algorithms, that combine advanced statistical methods and AI approaches, to provide a comprehensive view of cellular processes.
Our analytical pipeline is able to merge large datasets from diverse omic-layers (genomics, transcriptomics, proteomics, metabolomics, epigenetics), and extract valuable biological information that allows the identification of biomarkers and offers a deeper understanding of the disease mechanisms.

This makes our platform a valuable tool for precison medicine applications, enabling more accurate diagnostics and highly effective treatments.

For specific needs, please contact our Technology Transfer Manager

Structural determination and quantification of small molecules by RMN

We are able to study structural determination of samples in solution, of unknown compounds and confirm their structures; ligand-protein complexes study: molecule-molecule interaction analysis, and determination of molecular weights based on diffusion coefficients (DOSY).

Small molecules: Structural determination, quantitation by NMR (Ana Poveda and Tammo). We also screen fragment and molecular libraries towards target receptors.

We are expert in the application of modern NMR methods to study the structure of small and medium size molecules in solution, including unknown compounds. Our expertise includes organic molecules, natural products, peptides, saccharides and nucleotides. Our available methodologies permit to deduce or confirm the chemical structures of isolated molecules and to determine the chemical composition of mixtures, as well as the quantification and determination of their molecular weights, based on diffusion coefficients (DOSY NMR). We are also experts in performing detailed ligand-receptor (proteins, nucleic acids) molecular recognition studies.

Label free nLC MS/MS Quantitative Proteomics

Proteomics platform has incorporated one of the most sophisticated nLC MS/MS systems on market to address high sensitivity, performance and throughput label free quantitative proteomics studies. We can carry out different relative protein quantification projects such as inmunoprecipitations, subproteomes, cell or tissue extracts and biofluids. Besides, thanks to the incorporation of the EVOSEP new chromatographer, we can now tackle studies with large number of samples like the ones required in precision medicine projects.

Check Proteomic Platform

Post-Translation Modification Study

Proteomics Platform can characterize different post-translational modification by combining diverse sample preparation protocols and acquisition methods. The analysis of endogenous peptides from biofluids (e.g. tear, urine or endometrial fluid) or immunopeptidomes (HLA peptides) is also an area we are paying attention. Finally, thanks to the unprecedented sensitivity the systems achieves, we are currently conducting projects starting from very small amount of sample (e.g. tissue micro-dissections etc.)

Check Proteomic Platform

Single Particle quantitative analysis and 3D Electron

The most valuable single particle method based on CryoTEM. We are able to discriminate between EMPTY, FULL and semi-EMPTY particles (AAV, lentivirus, exosomes, nanoparticles) to be used as gene delivery system. The most advanced unsupervised methods of single particle quantitative and qualitative analysis on the market able to provide 3D reconstruction data of the gene delivery system of your choice.

The more advanced single particle quantitative analysis on the market, available at CIC bioGUNE Electron Microscopy Platform, is able to discriminate between empty, full-loaded and semi-empty particles to be used in gene therapy.

Our staff also provides a detailed set-up for your development process.

We are the best partner for set-up of your development process, and control of single particle production for pre-clinical and clinical applications.

Single Particle quantitative analysis and 3D Electron Tomography (Electron microscopy Platform

Experimental Design High Throughput Sequencing (HTS)

The Bioinformatics Unit at CIC bioGUNE is expert in the analysis of data obtained from different origins (proteomics, genomics, mRNseq, microbioma, etc.).

The results are presented in a customized manner, depending on the requirements of the client.

For specific needs, please contact our Technology Transfer Manager